UV-Vis Spectrophotometer Calibration: What Laboratories Should Know
UV-Vis spectrophotometer calibration is not one holmium scan. It is five independent checks — wavelength accuracy, photometric accuracy and linearity, stray light, spectral bandwidth, and baseline stability — each proved with a different certified reference and each carrying its own acceptance limit. This guide separates calibration from verification and qualification, shows why certified peak positions move with spectral bandwidth using the NIST SRM 2034 certificate values at 0.1, 1 and 3 nm, explains why ASTM E925 does not apply to instruments wider than 2 nm, compares the reference materials that actually prove each parameter, sets out interval logic against ILAC-G24 and intervention triggers, and lists the fields a calibration record needs to survive an audit.









