The BRT-5900UV UV Vis Spectrophotometer is a flagship double-beam research instrument featuring a Czerny-Turner (C-T) monochromator, an ultra-dense 1600 lines/mm holographic grating, and a high-sensitivity photomultiplier tube (PMT) detector. Engineered for advanced analytical applications, pharmaceutical R&D, high-precision chemical profiling, and life science research, it achieves an ultra-fine 0.05 nm wavelength resolution, exceptionally low stray light (≤0.015%T), and superior baseline flatness (≤±0.001 Abs) across the 190 nm to 900 nm spectral range with a tight 1.0 nm bandwidth, ±0.3 nm wavelength accuracy, and 0.1 nm wavelength repeatability.
Driven by dual deuterium and 20W tungsten halogen light sources with adjustable switching points, this high-stability UV Vis Spectrophotometer guarantees high transmittance accuracy (±0.3%T) and an extended dynamic range up to 4.000 Abs. Featuring an intuitive LCD dot-matrix screen, automatic startup wavelength calibration, internal storage for 200 datasets and standard curves, high-speed USB data export, and LPT parallel printing output, the 30 kg heavy-duty BRT-5900UV provides reproducible photometric, quantitative, and kinetic analysis.
UV Vis Spectrophotometer Measurement & Analysis Capabilities
This photomultiplier-equipped double-beam spectrophotometer enables advanced laboratory testing across a wide range of analytical methodologies:
| Analysis Mode |
Methodology & Operational Scope |
Primary Testing Application |
| High-Precision Photometric Testing |
Direct optical evaluations of Absorbance (-4.000 to 4.000 Abs) and Transmittance (0 to 200%T) at user-defined fixed wavelengths across 190–900 nm with 0.05 nm resolution |
High-density sample optical density determination, chemical raw material verification, and high-purity substance screening |
| Quantitative Concentration Profiling |
Concentration determinations using factor coefficients ($C = KA + B$) or multi-point linear calibration curves with automatic regression equation storage |
Active pharmaceutical ingredient (API) quantification, trace water pollutant assays, and complex chemical compound assays |
| PC-Driven Kinetics & Spectral Analysis |
Time-course dynamic tracking, multi-wavelength analysis, and full continuous spectral scanning using optional PC software via USB connection |
Enzymatic reaction rates, dynamic compound degradation studies, protein/nucleic acid characterization, and high-precision research assays |
Technological Features of BRT-5900UV UV Vis Spectrophotometer
- 1600 Lines/mm Grating & Photomultiplier Tube Detector
Combines a high-density 1600 L/mm holographic grating with a premium photomultiplier tube (PMT) detector for high light sensitivity, minimal baseline noise, and ultra-low stray light (≤0.015%T).
- Ultra-Fine Wavelength Resolution (0.05 nm)
Features an ultra-fine optical readout resolution of 0.05 nm with ±0.3 nm wavelength accuracy and 0.1 nm repeatability, allowing crisp peak separation in complex chemical solutions.
- Wide Dynamic Measuring Range (-4.000 to 4.000 Abs)
Supports an extended photometric linear range from -4.000 to 4.000 Abs, allowing direct testing of highly concentrated solutions without immediate dilution steps.
- Superior Baseline Flatness (≤±0.001 Abs) & Low Drift
The heavy-duty double-beam optical architecture delivers excellent baseline flatness (≤±0.001 Abs) and minimal drift, maintaining baseline stability during long research protocols.
- Dual Light Sources with Adjustable Switching Points
Configured with an imported deuterium lamp and a 20W tungsten halogen lamp featuring customizable switching points to maximize lamp longevity and optical stability.
- Dual Output Interfaces (USB PC Connection & LPT Parallel Port)
Provides digital signal output and a USB port for software integration and data transfer, alongside an LPT parallel port for direct hard-copy printing.
Technical Specifications of UV Vis Spectrophotometer
| Product Model |
BRT-5900UV High-Precision Double Beam UV Vis Spectrophotometer |
| Optical System & Monochromator |
Double Beam, C-T Monochromator, 1600 lines/mm Holographic Grating |
| Detector Type |
High-Sensitivity Photomultiplier Tube (PMT) Sensor |
| Wavelength Range & Resolution |
190 nm to 900 nm; Bandwidth: 1.0 nm; Wavelength Resolution: 0.05 nm |
| Wavelength Accuracy & Repeatability |
Accuracy: ±0.3 nm; Repeatability: 0.1 nm |
| Photometric Range & Stray Light |
0 to 200%T, -4.000 to 4.000 Abs; Transmittance Accuracy: ±0.3%T; Stray Light: ≤0.015%T |
| Baseline Flatness & Drift |
Baseline Flatness: ≤±0.001 Abs; Ultra-Low Baseline Drift |
| Light Sources & Sample Holder |
20W Tungsten Halogen Lamp & Deuterium Lamp; Fixed 2-Trench Sample Holder |
| Dimensions & Instrument Weight |
620 × 510 × 245 mm (L × W × H); Net Weight: 30 kg (66.14 lb) |
Standard Package Accessories Included
- Main Host Unit: BRT-5900UV Double Beam UV Vis Spectrophotometer (1 unit).
- Sample Cell Holder: Fixed 2-Trench Cuvette Holder.
- Quartz Cuvettes: 10 mm Standard Path Length Quartz Cuvettes (4 pcs for UV analysis).
- Glass Cuvettes: 10 mm Standard Path Length Glass Cuvettes (4 pcs for Visible spectrum analysis).
- Power & Data Connectivity: Dual-Voltage AC Power Cord, USB Cable.
- Documentation & Software: Operating Manual, Quality Certificate, Warranty Card.
Testing Workflow & Target Applications
Standardized Testing Workflow:
- Power on the BRT-5900UV; allow the system microcomputer to perform automatic lamp ignition, system zeroing, and startup wavelength calibration.
- Select Photometric or Quantitative testing modes via the LCD menu.
- Place a reference blank in the reference slot of the 2-trench holder to perform 0A / 100%T baseline zeroing across the target spectrum.
- Place the sample in the testing beam path to measure absorbance up to 4.000 Abs or calculate concentration values.
- Store datasets (up to 200 entries) in internal memory, transmit via USB to optional PC software for kinetics and scanning, or print hard copies via the LPT parallel port.
Target Customer Applications:
- Advanced pharmaceutical R&D laboratories, active pharmaceutical ingredient (API) testing centers, and QA/QC facilities.
- High-sensitivity environmental monitoring agencies (trace water pollutants, heavy metals, and COD assays).
- Molecular biology research institutes, biochemistry labs, and university chemistry departments.
- Chemical engineering centers, food purity inspection bays, and material science laboratories.
UV Vis Spectrophotometer FAQ
Q1: What are the main benefits of using a photomultiplier tube (PMT) detector in the BRT-5900UV?
A: Photomultiplier tube detectors offer higher sensitivity and signal-to-noise ratios compared to standard photocells. This enables high optical precision when measuring low-transmittance or highly absorbing samples, achieving stray light performance as low as ≤0.015%T.
Q2: How does the 1600 lines/mm grating improve optical performance?
A: The high-density 1600 lines/mm holographic grating delivers finer dispersion of light, enabling an ultra-fine wavelength resolution of 0.05 nm and a narrow 1.0 nm bandwidth for identifying close absorption peaks.
Q3: What analytical features are unlocked by connecting optional PC software via USB?
A: Connecting the instrument to PC software unlocks continuous full-spectrum wavelength scanning, real-time kinetic reaction rate monitoring, multi-wavelength testing, and customized GLP-compliant laboratory report generation.
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