BRT-5400UV UV Vis Spectrophotometer

The BRT-5400UV UV Vis Spectrophotometer is a high-throughput, split double-beam laboratory instrument engineered with a Czerny-Turner (C-T) monochromator, a 1200 lines/mm holographic grating, and an automated 8-cell changer system. Designed for high-sample-volume pharmaceutical QA/QC laboratories, environmental testing agencies, molecular biology institutes, and industrial research facilities, it delivers superior baseline flatness and long-term stability across the 190 nm to 1100 nm spectral range with a narrow 1.8 nm spectral bandwidth, ±0.5 nm wavelength accuracy, and ≤0.5 nm wavelength repeatability.

Equipped with dual deuterium and tungsten halogen light sources featuring customizable switching points, this automated UV Vis Spectrophotometer guarantees exceptional transmittance accuracy (±0.3%T) and low baseline drift. Featuring a 128×64 mm blue LCD screen, automatic startup wavelength calibration, onboard memory for 200 datasets and standard curves, high-speed USB data export, and LPT parallel printing output, the 17 kg BRT-5400UV dramatically elevates routine sample throughput and quantitative assay precision.

UV Vis Spectrophotometer Measurement & Analysis Capabilities

This automated double-beam spectrophotometer enables laboratory operators to execute streamlined photometric, quantitative, and kinetic testing routines with multi-sample efficiency:

Technological Features of BRT-5400UV UV Vis Spectrophotometer

• Automated 8-Cell Changer SystemFeatures an integrated automatic 8-position cell holder that drastically increases testing throughput, reducing manual sample switching and operational downtime.
• Split Double-Beam & C-T Monochromator ArchitectureCombines a split double-beam optical layout with a Czerny-Turner monochromator and 1200 lines/mm holographic grating for exceptional optical baseline stability and ultra-low stray light.
• Narrow 1.8 nm Bandwidth & Extended 190–1100 nm SpectrumDelivers a tight 1.8 nm optical bandwidth across the entire UV-Vis spectrum with 0.2 nm wavelength resolution, providing crisp peak resolution for complex samples.
• Dual Light Sources with Adjustable Switching PointsIncludes imported deuterium and tungsten halogen lamps with flexible, user-adjustable switching points and microcomputer control to extend lamp service life.
• Intelligent Startup Calibration & Real-Time Stability MonitoringAutomates wavelength calibration, 0A / 100%T zeroing upon startup, and continuously tracks optical drift to guarantee reproducible analytical results.
• Dual Output Ports (USB Host/PC & LPT Parallel Printer Port)Supports USB data export to PC analysis software or flash drives, along with an LPT parallel port for direct connection to dedicated laboratory printers.

Technical Specifications of UV Vis Spectrophotometer

Standard Package Accessories Included

• Main Host Unit: BRT-5400UV Double Beam UV Vis Spectrophotometer (1 unit).
• Sample Cell Holder: Automated 8-Position Cell Changer (installed).
• Quartz Cuvettes: 10 mm Standard Quartz Cuvettes (2 pcs included in standard set / expanded 4 pcs accessory configuration for UV analysis).
• Glass Cuvettes: 10 mm Standard Glass Cuvettes (4 pcs for Visible spectrum analysis).
• Power & Data Connectivity: Dual-Voltage AC Power Cable, USB Interface Cable.
• Documentation & Software: Operating Instruction Manual, Quality Certificate, Warranty Card.

Testing Workflow & Target Applications

Standardized Testing Workflow:

1. Power on the BRT-5400UV; the microcomputer executes lamp ignition, baseline zeroing, and wavelength self-calibration.
2. Select Photometric or Quantitative mode on the 128×64 mm blue LCD screen interface.
3. Load a blank reference cuvette into position 1 of the automated 8-cell changer to execute 0A / 100%T baseline calibration.
4. Fill remaining sample positions (up to 7 test samples) and initiate automatic sequential measurement for rapid batch concentration analysis.
5. Store data (up to 200 sets) in internal memory, export via USB to optional PC software for full-spectrum scanning and kinetics, or print reports via the LPT parallel port.

Target Customer Applications:

• Pharmaceutical manufacturing QA/QC and raw material batch inspection laboratories.
• Environmental water testing agencies (COD, nitrate, phosphate, and heavy metal assays).
• Molecular biology, biochemistry, and life science research institutes.
• University chemistry teaching labs, agricultural testing centers, and food safety inspection bays.

UV Vis Spectrophotometer FAQ

Q1: How does the automatic 8-cell changer improve laboratory workflow efficiency?

A: The automatic 8-cell changer allows technicians to load one blank reference and up to seven test cuvettes simultaneously. The instrument sequentially measures all samples without manual intervention, saving operational time and eliminating cell placement errors.

Q2: What is the main advantage of the split double-beam optical system in the BRT-5400UV?

A: The split double-beam optical system splits the light beam to continuously monitor optical energy. This offsets lamp fluctuations and environmental temperature changes, delivering outstanding baseline flatness and high measurement stability over long testing cycles.

Q3: What analytical functions are available when connecting optional PC software via USB?

A: While the standalone unit performs fast photometric and quantitative concentration testing, connecting optional PC software via USB unlocks full continuous spectrum scanning, dynamic time-course kinetics, multi-wavelength testing, and customized report formatting.